Actin localization in various rat tissues stained with DACM labeled heavy meromyosin.
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چکیده
منابع مشابه
The Use of Fluorescein-labeled Heavy Meromyosin for the Cytological Demonstration of Actin
The interaction of actin and myosin is considered to be a necessary condition for the contraction of striated muscle. A cytological demonstration that myosin can bind to a discrete part of the sarcomere, which from other evidence contains actin, was made by Huxley (1, p. 261). He observed an increase in phase contrast density when myosinextracted fibrils were treated wi th myosin in solution. H...
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Introduction: Beta-actin is a housekeeping gene, which is used as an internal standard in various biological studies. Recently, it was reported that the expression of commonly used housekeeping genes including ß–actin changes in disease states or under certain experimental conditions. Based on the reports about the effects of sex hormones on the expression of the ß- actin gene, in this study...
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Actin-like protein has been isolated from cultured since normal cell morphology is disrupted during sympathetic ganglia of chick (6) and mammalian glycerination, the exact localization of actinbrain (18), and accounts for up to 207 of the total HMM complexes in axons and cell bodies has not cell protein (6) . The exact ultrastructural localizabeen ascertained . tion of such a large amount of ac...
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The function of myosin A in the acceleration of actin polymerization was first observed by Laki and Clark (1) who noted the catalytic effect of myosin A. Martonosi and Gouvea (2) used a light scattering method to demonstrate that an enzymically active fragment of myosin A, heavy meromyosin, prepared by tryptic digestion, also accelerated the actin polymerization. Yagi and Sakakibara (3) have sh...
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Many microfilaments and microtubules are well preserved after glycerol-extraction of HeLa cells at room temperature (22 degrees ). Incubation in heavy meromyosin from rabbit skeletal muscle results in conspicuous and characteristic "decoration" of microfilaments of the contractile ring. Decoration is completely prevented by 10 mM ATP or 2 mM pyrophosphate, and fails to occur if heavy meromyosin...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1985
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.18.157